منابع مشابه
Replication arrest
Bacterial chromosomal replication forks are very efficient protein machines, moving along the template and synthesizing DNA at nearly 1000 bp/s. Replication by the two forks assembled at the origin continues at this rate for 40 min to copy the entire 4.7 Mb genome. Therefore, it is not surprising that these replication forks do not simply stop spontaneously when their task is complete. Bacteria...
متن کاملReplication Termination: Mechanism of Polar Arrest Revealed
The Tus-Ter protein-DNA complex of Escherichia coli blocks progression of DNA replication from only one direction at the replication terminus. As the replication fork helicase unwinds one side of Ter, a conserved cytosine flips out of the duplex and binds to Tus, thereby creating a locked complex that blocks the advancing helicase.
متن کاملDNA double-strand breaks caused by replication arrest.
We report here that DNA double-strand breaks (DSBs) form in Escherichia coli upon arrest of replication forks due to a defect in, or the inhibition of, replicative DNA helicases. The formation of DSBs was assessed by the appearance of linear DNA detected by pulse-field gel electrophoresis. Processing of DSBs by recombination repair or linear DNA degradation was abolished by mutations in recBCD ...
متن کاملSMC1-mediated intra-S-phase arrest facilitates bocavirus DNA replication.
Activation of a host DNA damage response (DDR) is essential for DNA replication of minute virus of canines (MVC), a member of the genus Bocavirus of the Parvoviridae family; however, the mechanism by which DDR contributes to viral DNA replication is unknown. In the current study, we demonstrate that MVC infection triggers the intra-S-phase arrest to slow down host cellular DNA replication and t...
متن کاملActivation of mammalian Chk1 during DNA replication arrest
Checkpoints maintain order and fidelity in the cell cycle by blocking late-occurring events when earlier events are improperly executed. Here we describe evidence for the participation of Chk1 in an intra-S phase checkpoint in mammalian cells. We show that both Chk1 and Chk2 are phosphorylated and activated in a caffeine-sensitive signaling pathway during S phase, but only in response to replic...
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ژورنال
عنوان ژورنال: Cell
سال: 1995
ISSN: 0092-8674
DOI: 10.1016/0092-8674(95)90504-9